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p-HSF1 (S303) pAb

p-HSF1 (S303) pAb

Cat no: BS4795


Supplier: Bioworld Technology, Inc.
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Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
Catalogue number: BS4795
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: Q00613
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Serine 303.
Species: p-HSF1 (S303) pAb detects endogenous levels of HSF1 protein only when phosphorylated at Ser303.
Molecular weight: ~ 82 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSF1; HSTF 1; HSTF1;
Additional info: For research use only, not for use in diagnostic procedure.

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