p-IkB-(alpha) (Y42) pAb

Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase (alpha) (IKK(alpha)), previously designated CHUK, interacts with IkB-(alpha) and specifically phosphorylates IkB-(alpha) on the sites that trigger its degradation Serines 32 and 36. IKK(alpha) appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK(alpha) is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK(alpha), IKK(beta) and IKK(gamma) , and each appear to make essential contributions to IkB phosphorylation.

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SPECIFICATIONS

Catalog Number

BS4736

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic phosphopeptide derived from human IkB-(alpha) around the phosphorylation site of Tyrosine 42.

Species

p-IkB-(alpha) (Y42) pAb detects endogenous levels of IkB-(alpha) protein only when phosphorylated at Tyr42.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P25963

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 36 kDa

Alternative Names

I kappa B alpha; I kappa B alpha; I(Kappa)B(alpha); I(Kappa)B(alpha); I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBA; IKBA; IKBalpha; MAD 3; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; Major histocompatibility complex enhancer binding protein MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBI; NFKBIA; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B c