p-IPP2 (S120/S121) pAb

Two inhibitors of protein phosphatase 1 (PP1) include the phosphatase inhibitor 1 (IPP-1) and phosphatase inhibitor 2 (IPP-2) . IPP-2, also known as I-2, interacts with the catalytic subunit of PP1 to form the heterodimer PP1I. The PP1I complex is present in the cytosol of cells in a broad range of vertebrate and invertebrate species. Although the heterodimer itself is inactive, a reversible phosphorylation of IPP-2 at Thr 72 by glycogen-synthase-kinase (GSK3) initiates activation of the heterodimer complex in vitro. Phosphorylation of IPP-2 by casein kinase-II at Ser 86, Ser 120, and Ser 121 enhances the rate of phosphorylation by GSK3 at Thr 72 and effectively activates the heterodimer complex. Besides moderating PP1 activity, IPP-2 may play a role as a chaperone for the correct folding of PP1.

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SPECIFICATIONS

Catalog Number

AP0341

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic phosphopeptide derived from human IPP2 around the phosphorylation site of Serine 120 and 121.

Species

p-IPP2(S120/S121) pAb detects endogenous levels of IPP2 protein only when phosphorylated at Ser120/S121.

Dilutions

WB:1:500~1:1000

Swiss Prot

P41236

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 30 kDa

Alternative Names

IPP 2; IPP-2; IPP2; IPP2; MGC87148; Phosphatase inhibitor 2; Phosphoprotein phosphatase; Ppp1r2; PPP1R2 protein; Protein phosphatase 1 regulatory (inhibitor) subunit 2; Protein phosphatase 1 regulatory subunit 2; Protein phosphatase inhibitor 2;