p-PKC-pan ((alpha)T497) pAb

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms ((alpha), (beta)I, (beta)II and (gamma)) and novel (n) PKC isoforms ((delta), E, ?, n and 0). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC (delta) and E are independent of Ca2+.

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SPECIFICATIONS

Catalog Number

BS4348

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Threonine 497.

Species

p-PKC-pan ((alpha)T497) pAb detects endogenous levels of PKC PKC (alpha), (beta) I, (beta) II, (gamma), (delta), E, n, (zeta)and 0 isoforms only when phosphorylated at a residue homologous to threonine 497 of human PKC(alpha).

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P17252/P05771/Q05655/Q02156/P05129/P24723/Q04759/Q05513

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~75 to 88 kDa

Alternative Names

Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA; Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB; Protein kinase C gamma type; PKC-gamma; PKCG; PRKCG; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Sphingosine-dependent protein kinase-1; SDK1; PRKCD; Protein kinase C epsilon type; nPKC-epsilon; PRKCE; PKCE; Protein kinase C zeta type; nPKC-zeta; PRKCZ; PKC2; PKC zeta; Protein kinase C theta type; nPKC-theta; PRKCQ; PRKCT