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p-Raf-B (T599) pAb

p-Raf-B (T599) pAb

Cat no: AP0304


Supplier: Bioworld Technology, Inc.
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Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.
Catalogue number: AP0304
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug/100ul
Swiss prot: P15056
Dilutions: WB: 1:500~1:1000
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic phosphopeptide derived from human Raf-B around the phosphorylation site of Threonine 599.
Species: p-Raf-B (T599) pAb detects endogenous levels of Raf-B protein when phosphorylated at Thr599.
Molecular weight: ~ 80 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: FLJ95109; 94 kDa B raf protein; B raf 1; B raf 1; B Raf proto oncogene serine threonine protein kinase; B Raf proto oncogene serine threonine protein kinase; BRAF 1; BRaf; BRaf; BRAF; BRAF1; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; p94; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; RAFB 1; RAFB1; RAFB1; Serine/threonine
Additional info: For research use only, not for use in diagnostic procedure.

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