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p-Separase (S801) pAb

p-Separase (S801) pAb

Cat no: BS4462


Supplier: Bioworld Technology, Inc.
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Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, Separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, Separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active Separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.
Catalogue number: BS4462
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: Q14674
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic phosphopeptide derived from human Separase around the phosphorylation site of Serine 801.
Species: p-Separase (S801) pAb detects endogenous levels of Separase protein only when phosphorylated at Ser801.
Molecular weight: ~ 230 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: ESP 1; ESP1; ESPL 1; ESPL1; Extra spindle poles like 1; Extra spindle poles like 1 protein; FLJ46492; KIAA0165; Separin; Similar to fission yeast cut1and gene; SSE;
Additional info: For research use only, not for use in diagnostic procedure.

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