p27 Complex, Activated, Recombinant, Human (p27kip1, Cyclin-dependent kinase inhibitor 1B, Kip1)

p27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.\n\nSource:\nRecombinant corresponding to full length human p27, N-terminal c-Myc-tagged, 6His-tagged, recombinant human Cyclin E1 full length and untagged, recombinant human CDK2 full length, co-expressed by baculovirus in Sf21 insect cells.\n\nMolecular Weight:\n~34kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029571

Size

10ug

Form

Supplied as a liquid in in 25mM MOPS pH7.5, 5mM MgCl2, 0.01% Tween 20, 1mM ATP.

Purity

~94.6% (SDS-PAGE and Coomassie blue staining)

References

1. Besson A. et al. Discovery of an Oncogenic Activity in p27Kip1 that Causes Stem Cell Expansion and a Multiple Tumor Phenotype. Genes Dev., 21: 1731-1746, 2007. 2. Grimmler M. et al. Cdk-Inhibitory Activity and Stability of p27Kip1 are Directly Regulated by Oncogenic Tyrosine Kinases. Cell, 128: 269-280, 2007. 3. Lee J. and Kim S. S. The Function of p27Kip1 During Tumor Development. Exp Mol Med. 41: 765-771, 2009. 4. Ungermannova D. et al. Ubiquitination of p27Kip1 Requires Physical Interaction with Cyclin E and Probable Phosphate Recognition by SKP2. J. Biol. Chem., 280: 30301-30309, 2005. 5. Pellegata N. S. et al. Germ-line Mutations in p27Kip1 Cause a Multiple Endocrine Neoplasia Syndrome in Rats and Humans. PNAS, 103: 15558-15563, 2006.