P27 (Cyclin-dependent kinase inhibitor p27, p27Kip1)

Cyclin-dependent kinase inhibitor 1B (CDKN1B), also known as p27 (KIP1), is a cyclin-dependent kinase (Cdk) inhibitor implicated in G1 phase arrest, which negatively regulates G1 phase progression in response to TGF beta and represents a tumor suppressor gene. Human p27 gene is mapped to chromosome 12p12.3 p27 can be both an inhibitor and a substrate of cyclin E-CDK2. p27, abundant in quiescent cells and drops after serum stimulation, plays a role in mediating VSMC hypertrophy. p27 degradation is subject to dual control by the accumulation of both SKP2 and cyclins following mitogenic stimulation. It regulates cell proliferation by binding to and modulating the activity of cyclin-dependent kinases. Reduced p27 activity is fundamental for the development of many human malignancies including breast, prostate, colon and gastric carcinomas.\n\nUniProt Number:\nP46527\n\nGene ID:\nCDKN1B\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

145021

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Martin E, Cacheux V, Cave H, Lapierre JM, Le Paslier D, Grandchamp B.Localization of the CDKN4/p27Kip1 gene to human chromosome 12p12.3. Hum Genet. 1995 Dec; 96(6):668-70.\n2. Sheaff RJ, Groudine M, Gordon M, Roberts JM, Clurman BE.Cyclin E-CDK2 is a regulator of p27Kip1. Genes Dev. 1997 Jun 1; 11(11):1464-78.\n3. Braun-Dullaeus RC, Mann MJ, Ziegler A, von der Leyen HE, Dzau VJ.A novel role for the cyclin-dependent kinase inhibitor p27(Kip1) in angiotensin II-stimulated vascular smooth muscle cell hypertrophy. J Clin Invest. 1999 Sep; 104(6):815-23.\n4. Carrano AC, Eytan E, Hershko A, Pagano M.SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat Cell Biol. 1999 Aug; 1(4):193-9.\n5. Gopfert U, Kullmann M, Hengst L.Cell cycle-dependent translation of p27 involves a responsive element in its 5'-UTR that overlaps with a uORF. Hum Mol Genet. 2003 Jul 15; 12(14):1767-79.

Additional Info

Recognizes human, mouse and rat P27. No crossreactivity with other proteins.