Home  >  Products  >  P4HA1 (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)
P4HA1 (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)

P4HA1 (P4HA, Prolyl 4-hydroxylase Subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase Subunit alpha-1)

Cat no: P0750-50C

Supplier: United States Biological
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This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1ug/ml, observed in lysates of cell lines HEK293, A431 and NIH3T3 on ~60kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: P0750-50C
Reactivities: Human, Mouse, Rat, Canine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI. Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer. J Biol Chem. 1997 Jul 11;272(28):17342-8.
Additional info: Recognizes human P4HA1. Species sequence homology: Canine, mouse and rat.

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