p66 alpha (MeCP1 Repressor Component, Methylation of Cytosine at CpG Dinucleotide)

Methylation of cytosine at CpG dinucleotides is a common feature of many higher eukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histone deacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism of methylated DNA silencing. P66, a zinc finger-containing protein is one of the components of the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different forms of the same zinc finger-containing protein and are conserved from C. elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 and CR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci(4). \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\npositive control: HeLa or Raw whole cell lysate \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P1001-35D

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS containing 0.02% sodium azide and 0.1 mg/ml BSA.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Zhang, Y., Ng, H., Erdjument-Bromage, H., Tempst, P., Bird, A., and Reinberg, D. Genes Dev 13:1924-1935 (1999). \n2. Xue, Y., Wong, J., Moreno, G., Young, M., Cote, J., and Wang, W. Mol. Cell 2:851-861 (1998). \n3. Tong, J., Hassig, C., Schnitzler, G., Kingston, R., Schreiber, S. Nature 395:917-921 (1998). \n4. Feng, Q., Cao, R., Xia, L., Erdjument-Bromage, H., Tempst, P., and Zhang, Y. Mol. Cell Biol 22:536-546 (2002). \n5. Dignam, J.D., et al., Nucl. Acids Res. 11: 1475, 1983 Feng, Q., et al., Mol. Cell Biol. 22: 536, 2002.

Additional Info

Methylation of cytosine at CpG dinucleotides is a common feature of many higher eukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histone deacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism of methylated DNA silencing. P66, a zinc finger-containing protein is one of the components of the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different forms of the same zinc finger-containing protein and are conserved from C. elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 and CR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci(4). \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\npositive control: HeLa or Raw whole cell lysate \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C or colder. Aliquots are stable for at least 24 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.