PA28, alpha (Proteasome Activator 28-alpha subunit)

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionary conserved subunits of PA28; PA28a (PSME1), PA28b (PSME2) and PA28g (PSME3) (1,2). PA28a and PA28b form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28a/b complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptide from foreign proteins (2). PA28g exists in the form of a homoheptamer and is mainly located in the nucleus. PA28g complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P1700-20

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

References

1 Dahlmann, B. (2005) Essays Biochem. 41, 31-48. \n 2 Rechsteiner, M. and Hill, C.P. (2005) Trends Cell Biol. 15, 27-33. \n 3 Zhou, P. (2006) Cell 124, 256-257. \n 4 Li, X. et al. (2006) Cell 124, 381-392.

Additional Info

Detects endogenous levels of total PA28 alpha protein.