PA28, alpha (PSME, Proteasome Activator 28-alpha subunit)

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.\n\nAlternate Names: anti-PSME1 antibody, anti-proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody, anti-IFI5111 antibody, anti-MGC8628 antibody, anti-PA28A antibody, anti-PA28alpha antibody, anti-REGalpha antibody, anti-11S regulator complex alpha subunit antibody, anti-29-kD MCP activator subunit antibody, anti-activator of multicatalytic protease subunit 1 antibody, anti-interferon gamma up-regulated I-5111 protein antibody, anti-interferon-gamma IEF SSP 5111 antibody, anti-interferon-gamma-inducible protein 5111 antibody, anti-proteasome activator subunit 1 antibody, anti-proteasome activator subunit-1 antibody \n \nApplications: \nSuitable for use in ELISA, Western Blotting. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:50,000-1:65,000\nWestern Blotting: 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P1700-21

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in tris-saline [pH7.3] with 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Brychcy M, Kuckelkorn U, Hausdorf G, Egerer K, Kloetzel PM, Burmester GR, Feist E. Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28. Arthritis Rheum. 2006 Jul;54(7):2175-83. PMID: 16802355

Additional Info

Expected to recognize human PSME isoform 1 (NP_006254.1). Species Crossreactivity: Expected based upon sequence homology: canine, mouse, rat