PAK4, phosphorylated (pS474), PAK5 (pS602), PAK6 (pS560)

p21-activated kinase 4 (PAK4) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1, 2). The PAK proteins can be essentially divided into two categories, group I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK4 has been shown to regulate cytoskeletal organization and protect cells from apoptosis induced by multiple stimuli, including serum withdrawal, tumor necrosis factor (TNF) treatment, or UV irradiation (4). PAK4 expression is elevated in many cancer cell lines, and activated PAK4 is highly transforming, suggesting an important role in tumorigenesis (5).\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000-1:10,000\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

P2488-25

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

References

1. Manser, E., Leung, T., Salihuddin, H., Zhao, Z., and Lim, L. (1994) Nature 363, 364-367 2. Daniels, R. H., and Bokoch, G. M. (1999) Trends Biochem. Sci. 24, 350-3553. Jaffer, Z. M., and Chernoff, J. (2002) Int. J. Biochem. Cell Biol. 34, 713-717 4. Gnesutta, N., Qu, J., and Minden, A. (2001) J. Biol. Chem. 276, 14414-14419 5. Qu, J., Cammarano, M. S., Shi, Q., Ha, K. C., de Lanerolle, P. and Minden, A. (2001). Mol. Cell. Biol. 21, 3523-3533.

Additional Info

Recognizes human PAK4 phosphorylated at Serine 474, PAK5 phosphorylated at Serine 602, and PAK6 phosphorylated at Serine 560. Species crossreactivity: Mouse, rat.