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PCNA mAb

PCNA mAb

Cat no: MB0156

Supplier: Bioworld Technology, Inc.
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The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
Catalogue number: MB0156
Reactivities: Human, Mouse, Rat, Hamster, Non-Human Primate
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ug/100ul
Swiss prot: P12004
Dilutions: WB: 1:1000 ICC: 1:50~100 IP: 1:50~100
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Recombinant full length Human PCNA.
Species: This antibody detects endogenous levels of PCNA and does not cross-react with related proteins
Molecular weight: Predicted band size:36KDa Observed band size:36KDa
Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: Proliferating cell nuclear antigen; Cyclin
Additional info: For research use only, not for use in diagnostic procedure.

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