PCTAIRE 1

Three distinct PCTAIRE isoforms (PCTAIRE 1, PCTAIRE 2 and PCTAIRE 3) have been identified in humans and belong to the Cdk family of serine/threonine protein kinases. These proteins have a core kinase domain flanked by unique amino-and carboxy- terminal domains. Cdk proteins are known to regulate the cell cycle. All three PCTAIRE isoforms are abundantly expressed and catalytically active in post-mitotic brain, suggesting that they may function in processes other than cell division (1). PCTAIRE 1 is a cytoplasmic phosphoprotein whose kinase activity peaks in G2 and S phase (2). While one study indicates that noncovalent interactions with a regulatory component (such as a cyclin) are necessary for catalytic activity of PCTAIRE 1, others show that the monomeric protein is fully active (3). The Cdk5/p25 complex phosphorylates PCTAIRE 1 at Ser95, enhancing its kinase activity (4). \n\nApplication:\nSuitable for use in Western Blot, Flow Cytometry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nFlow Cytometry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3120-08

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied in 10mM sodium HEPES, pH 7.5,, 150 mM NaCl, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1. Meyerson, M., et al., EMBO J. 11: 2909- 2917 (1992). 2.Charrasse, S., et al., Cell Growth Differ. 10: 611-620 (1999). 3 Graeser, R., et al., J. Cell Sci. 115: 3479-3490 (2002). 4. Cheng, K., et al., J. Biol. Chem. 277: 31988-31993 (2002).

Additional Info

Detects endogenous levels of total PCTAIRE 1. Species Crossreactivity: mouse. Species sequence homology: human and rat.