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PDE3A, Active, Recombinant, Human, His-GST-Tag (cGMP-inhibited 3',5'-cyclic Phosphodiesterase A, Cyclic GMP-inhibited Phosphodiesterase A, CGI-PDE A)

Cat no: 137335

PDE3A, Active, Recombinant, Human, His-GST-Tag (cGMP-inhibited 3',5'-cyclic Phosphodiesterase A, Cyclic GMP-inhibited Phosphodiesterase A, CGI-PDE A)

Phosphodiesterase 3A is a cGMP inhibited, cAMP/cGMP dual-specific phosphodiesterase. PDE3A has been implicated in male erectile dysfunction, mammalian oocyte maturation, platelet function, vascular smooth muscle function, and salivary gland tumors. Three alternatively spliced transcripts have been reported.\n\nSource:\nRecombinant corresponding to aa669-1141 from human PDE3A, fused to His-GST-tag at N-terminal, expressed in a Baculovirus infected Sf9 cell expression system.\n\nMolecular Weight:\n~82kD\n\nApplications:\nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nSpecific Activity:\n~4000pmol/min/ug\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137335

Size

5ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 16mM glutathione, 20% glycerol, 3mM DTT.

Purity

Purified (~82%)

References

1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006) 2. Hambleton,R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

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