PDGF Receptor alpha/beta, phosphorylated (Tyr849/Tyr857)

The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor alpha (PDGFRa) and PDGF receptor beta (PDGFRb). PDGFRa and PDGFRb share 75%-85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27%-28%) of homology. PDGFRa homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRb homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGFa/b receptor binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer. PDGFRa and PDGFRb can each form heterodimers with EGFR, which is also activated by PDGF. Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding. Ligand binding induces receptor dimerization and auto-phosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration and differentiation. Tyr751 in the kinase-insert region of PDGFRb is the docking site for PI3 kinase. Phosphorylated pentapeptides derived from Tyr751 of PDGFRb inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRb. Tyr740 is also required for PDGFRb mediated PI3 kinase activation.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4258-21Y

Size

100ul

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Deuel, T.F. et al. (1988) Biofactors 1, 213-217. 2. Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516. 3. Betsholtz, C. et al. (2001) Bioessays 23, 494-507. 4. Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45. 5. Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38. 6. Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272. 7. Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272. 8. Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382.

Additional Info

Recognizes endogenous levels of human PDGF receptor alpha and beta at ~190kD only when phosphorylated on Tyr849 of PDGFR alpha and Tyr857 of PDGFR beta. May crossreact with other activated tyrosine kinases. Species Crossreactivity: Mouse, rat