PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes)

PEA-15 (Phosphoprotein enriched in astrocytes of 15kD) is a 15kD cytoplasmic protein that regulates cell proliferation and apoptosis. It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes. Human PEA-15 is 130aa in length. It contains a death effector domain (aa3-81), plus two regulatory Ser phosphorylation sites. In a quiescent cell, it is either non-phosphorylated or constitutively phosphorylated at Ser116. Non-phosphorylated PEA-15 binds ERK, inhibiting cell proliferation. Phosphorylation at Ser116 promotes PEA-15 binding to \nFADD, blocking apoptosis. And activation-induced phosphorylation at Ser104 blocks PEA-15 binding to ERK, promoting cell proliferation. There is one potential alternate start site 21aa upstream of the standard start site, and a second isoform that shows an Asn substitution for aa 36-58. Full-length human and mouse PEA-15 show 99% aa identity. \n \nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1.0ug/ml\nWestern Blot: 1.0ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts