Peanut, BioAssay(TM) ELISA Kit (Arachis hypogaea)

Peanut (Arachis hypogaea) belongs to the legume family. With 25 % the fraction of proteins in peanuts is very high. Many of these proteins are known for being allergenic, such as Arachins and Conarachins which are contained in relative high amounts. For this reason peanut represents one of the most important food allergens. For peanut allergic persons hidden peanut allergens in food are a critical problem. Already very low amounts of peanuts can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, peanut allergic persons must strictly avoid the consumption of peanuts or peanut containing food. Cross-contamination, mostly in consequence of the production process is often noticed. The chocolate production process is a representative example. This explains why in many cases the existence of peanut residues in foods cannot be excluded. For this reason sensitive detection systems for peanut residues in foodstuffs are required. The United States Biological Peanut ELISA represents a highly sensitive detection system and is particularly capable of the quantification of peanut residues in cookies, cereals, ice cream and chocolate.\n\nIntended Use:\nFor the quantitative determination of peanut in food.\n\nSensitivity: \n0.1ppm\n\nRecovery (spiked samples): ~90-110%\n\nIncubation Time: 60 min\n\nTest Principle:\nThe United States Biological Peanut quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against peanut proteins is bound on the surface of a microtiter plate. Peanut containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugated second antibody directed against peanut proteins is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450nm. The concentration of peanut is directly proportional to the color intensity of the test sample.\n\nKit Components:\n1. Microtiter plate 1x96 wells each, coated with anti-peanut antibodies.\n2. Standard 0ng/ml 1x1ml, ready to use.\n3. Standard 1ng/ml 1x1ml, ready to use.\n4. Standard 4ng/ml 1x1ml, ready to use.\n5. Standard 10ng/ml 1x1ml, ready to use.\n6. Standard 40ng/ml 1x1ml, ready to use.\n7. Conjugate (anti-peanut-peroxidase): 1x15ml, ready to use.\n8. Substrate Solution (TMB): 1x15ml, ready to use.\n9. Stop Solution (0.5M H2SO4): 1x15ml, ready to use.\n10. Extraction and sample dilution buffer 10x (Tris): 2x120ml.\n11. Washing Solution 10x (PBS + Tween 20): 1x60ml. \nDilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up to 37 degrees C for 15 minutes.\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3129-86

Size

1Kit

Applications

ELISA

References

1. Sen M, et al. (2002) - Protein structure plays a critical role in peanut allergen stability and may determine immunodominant IgE-binding epitopes. J Immunol, 169:882-87. 2. Turcanu V, et al. (2003) - Characterization of lymphocyte respones to peanuts in normal children, peanut allergic children, and allergic children who acquired tolerance to peanuts. J Clin Invest, 111(7):065-72. 3. Koppelmann SJ, et al. (2001) - Quantification of major peanut allergens Ara h 1 and Ara h 2 in the peanut varieties. Allergy 56(2):132. 4. Pom