Pericentrin 1 (PCNT1, PCN, PCNT, Pericentrin 1, Frount, Nucleoporin Nup75, Nucleoporin 85kD, NUP85)

Alternate Name: PCNT1, PCN, PCNT, pericentrin 1, frount, nucleoporin Nup75, nucleoporin 85kDa, NUP85\n\nDomain Information: Chitinase\n\nGene Ontology Terms: \n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 75kD band observed in HeLa lysates (calculated MW of 75kD according to NP_079120). Recommended concentration: 1-3ug/ml.\n\n1ug/ml staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See P3340-50-P. Antibody

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SPECIFICATIONS

Catalog Number

P3340-50

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M. , Pericentrin, a highly conserved centrosome protein involved in microtubule organization., Cell. 1994 Feb 25;76(4):639-50.

Additional Info

Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KD band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies). Species Crossreactivity: human, mouse, rat.