Pericentrin (Kendrin, Pericentrin-B, PCNT, KIAA0402, PCNT2)

Pericentrin binds to calmodulin and is expressed in the centrosome that serves as a multifunctional scaffold for anchoring proteins and protein complexes. Evidence suggests that pericentrin plays a role in microtubule nucleation and spindle organization. Recent studies show pericentrin plays a role in Majewski/micorcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel Syndrome. Recent use in network modeling indicates a direct link between breast cancer susceptibility to centrosome defects. Pericentrin is a potential marker for centrosome defects in cancer and cancer progression, as centrosomal abonormalities in solid tumors are accompanied by increased pericentrin levels and defects in pericentrin organization.\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nImmunocytochemistry: 1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

029579

Size

100ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Delaval, B., et al. (2010). J Cell Biol. 188(2): 181-190. 2. Griffith E., et al. (2008). Nat Genet. 40(2): 232- 236. 3. Rauch, A., et al. (2008). Science. 319(5864): 816-819. 4. Pujana, M.A., et al. (2007). Nat Genet. 39(11): 1338-1349. 5. Wen, Y., et al. (2008). J Biol Chem. 283(5): 2793-2803.

Additional Info

Recognizes human Pericentrin. Species sequence homology: Mouse (89%).