Perilipin

Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin A and B arise by alternative RNA splicing from a single gene, generating polypepides of 57 and 46kD, respectively. The N-terminus, however, remains unchanged. The antiserum recognizes perilipins (A and B) \nlocated at the surface of intracellular storage lipid droplets present, e.g. in the adrenal gland, adipocytes of white and brown adipose tissue, and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. \n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot (ECL): 1:2000\nImmunohistochemistry: 1:100-1:200 for 1 hour at RT on paraffin sections after microwave treatment or on frozen sections.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3340-99

Size

100ul

Applications

IHC, WB

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Blanchette-Mackie EJ, et al. J Lipid Res 36(6), 1211-1226 (1995). 2. Souza SA, et al. JBC 273(38), 24665-24669 (1998). 3. Heid HW, et al. Cell Tissue Res 294, 309-321 (1998). 4. Martinez-Botas J, et al. Nat. Genet. 26, 474-479 (2000). 5. Wolins NE, et al. J. Biol. Chem. 276, 5101-5108 (2001). 6. Ryde?n M, et al. Biochem Biophys Res Com 318, 168-175 (2004). 7. Opretzka, ME: Topographie und Dynamik von Perilipin und Adipophilin in Lipidtropfen. PhD Thesis, University of Muenster/Germany (2005). 8. Forcheron F, et al. Arterioscler Thromb Vasc Biol 25, 1-7 (2005). 9. Yamaguchi T, et al. Mol Cell Biochem 284, 167-173 (2006). 10. Muthusamy K, et al. Immunohistochemical staining for adipophilin, perilipin and TIP47 in ocular sebaceous gland carcinoma. J Clin Pathol (2006).

Additional Info

Recognizes perilipins (A and B) at ~57 and ~46kD, respectively. Does not cross-react with ADRP (adipophilin) or TIP47 proteins. Species Crossreactivity: human, rat and mouse