Perilipin (PERI, PLIN, Lipid Droplet-associated Protein)

The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis.\n\nPositive Control: Human Adipose lysate\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:32,000. \nWestern Blot: 0.01-0.03ug/ml. ~ 55-60kDa band observed in Human Adipose lysates (calculated MW of 56.0kDa according to NP_002657.2).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3340-98A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Tris-saline [pH7.3] with 0.5% BSA

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Wolins NE, et al. A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Lett. 2006 Oct 9;580(23):5484-91. Epub 2006 Sep 1. Review. PMID: 16962104

Additional Info

Species Crossreactivity: Human. Other species not tested. Expected based upon sequence homology: Rat, canine