Period Clock Protein 1 (Period 1, Period-1, PER1, Period Circadian Protein Homolog 1, Period Drosophila Homolog of, Period Homolog 1, Circadian Clock Protein PERIOD1, Circadian Clock Protein PERIOD-1, Circadian Pacemaker Protein RIGUI, HGNC:8845, hPER, hP

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence (IC): 1:10-1:2000\nWestern Blot: 1:200\nImmunohistochemistry (Frozen, paraffin): 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3342-02A

Size

200ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Darlington TK et al. Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per tim. Science 280:1599-603 (1998). 2. Gekakis N et al. Role of the CLOCK protein in the mammalian circadian mechanism. Science 280:1564-9 (1998). 3. Reppert SM A clockwork explosion! Neuron 21:1-4 (1998).

Additional Info

Recognizes mouse Period Clock Protein 1, as well as recombinant mouse Period Clock Protein 1. Species Crossreactivity: human