Period Clock Protein (dPER)

Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Drosophila Period clock protein is involved in the generation of biological rhythms. It interacts with Timeless protein (Tim). dPER first accumulates in the perinuclear region about an hour before it is transported to the nucleus. It contains a remarkable run of alternating Gly-Thr residues.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000 using ECL\nELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide:\nP3342-01A: Period Clock Protein 1 (dPER) Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P3342-01B

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Dro/Arth

Form

Supplied as a liquid with PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Jackson, F.R., et al. (1986) Nature 320: 185. 2. Reddy, P., et al. (1986) Cell 46: 53. 3. Kilman, R.M., et al. (1993) Genetics 133: 375. 4. Citri, Y., et al. (1987) Nature 326: 42.

Additional Info

Recognizes Drosophilla Per. The epitope is conserved in various alternatively spliced forms of dPer (dPerA, dPerB, etc.). Species Crossreactivity: Drosophilla peptide sequence has no sequence homology with mammalian PER or Clock proteins.