Peripherin (PRPH, Neurofilament 4, NEF4)

Peripherin is a 57kD Class III intermediate filament subunit found initially in sensory neurons of the peripheral nervous system, which gives the protein its name. Certain sensory neurons in the brain stem express Peripherin normally, as do a few cortical and hippocampal neurons. Interestingly, peripherin protein is upregulated in the lower motor neurons that degenerate in Amyotrophic Lateral Sclerosis (ALS), focusing attention on peripherin as a potential contributory factor to this serious human neurodegenerative disease. Peripherin is also expressed in certain neuroendocrine tumors and in the insulinproducing cells of the pancreas.\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:500\nWestern Blot: 1:2500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3345-01C

Size

100ug

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Fel, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Keilhauer CN, et al. Clinical Findings in a Multigeneration Family With Autosomal Dominant Central Areolar Choroidal Dystrophy Associated With an Arg195Leu Mutation in the Peripherin/RDS Gene. Arch. Ophthalmol. Jul 2006; 124(7): 1020-1027. 2. Lee ES, et al. Characterization of peripherin/rds and rom-1 transport in rod photoreceptors of transgenic and knockout animals. Invest. Ophthalmol. Vis. Sci. May 2006; 47(5): 2150-2160.

Additional Info

Recognizes rat Peripherin Species crossreactivity: human, rabbit, feline and mouse.