Peripherin (PRPH, PRPH1, Neurofilament 4, NEF4)

Perpherin is an intermediate filament protein expressed in peripheral neurons including enteric ganglian cells and it is present in neuronal derivatives of the neural crest. This antibody does not stain chromaffin cells, which is an advantage over other neural markers, but is a useful marker for neural crest derived tumors such as neuroblastomas and ganglioneuroblastomas for research applications.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50-1:100 for 1 hr at RT\nImmunohistochemistry: 1:100-1:200 for 1 hr at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nSmall bowel and neuroblastoma; peripheral nerves and ganglion cells (cytoplasmic staining pattern)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3345-04B

Size

1ml

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG2b

References

1.Szabolcs M J, Visser J, Shelanski M L, et al.. Peripherin: A novel marker for the immunohistochemical study of malformations of the enteric nervous system. Pediatric Pathology & Laboratory Medicine. 16:51-70 (1996). 2.Foley J, Witte D, Chiu F-C, et al.. Expression of the neural intermediate filament proteins peripherin and neurofilament-66/a-internexin in neuroblas- toma. Laboratory Investigation. 71(2):193-199 (1994). 3.Kajiwara K, Berson E L and Dryja T P. Digenic retinitis pigmentosa due to mutations at the unlinked peripherin/rds and rom1 loci. Science. 264:1604-1608 (1994).

Additional Info

Recognizes Peripherin. Species Crossreactivity: Human.