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Peroxidase Anti-Peroxidase (PAP) Soluble Complex

Cat no: P3350-29

Peroxidase Anti-Peroxidase (PAP) Soluble Complex

The peroxidase-antiperoxidase (PAP) approach utilizes a complex of enzyme and antibody to facilitate amplification of signal, indirectly detecting the amount of primary antibody used in an assay. Generated in Rabbit, the PAP complex can be utilized in any assay that utilizes a rabbit primary antibody. With the addition of a secondary antibody that recognizes rabbit, the reporter PAP complex allows for amplification of the luminescent signal (as three molecules of enzyme are bound to every two immunoglobulins).\n\nApplications:\nSuitable for use in Immunohistochemistry and Peroxidase Enzymatic Labeling. Other applications have not been tested\n\nRecommended Dilutions:\nNote: Recommended that further dulutions be made with 5% normal serum from same host bridging antibody.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

P3350-29

Size

5mg

Applications

IHC

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2. No stabilizing proteins or preservatives added. Labeled with HRP.

P Type

Pab

Purity

Purified by delipidation, salt fractionation and ion exchange chromatography.

Isotype

IgG

Additional Info

Recognizes peroxidase. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum and anti-peroxidase.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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