Peroxiredoxin 6 (PRDX-6, Prdx6-rs3, AA690119, 1-Cys Peroxiredoxin, 1-Cys-PRX, 1-CysPrx, 9430088D19Rik, Acidic Calcium-independent Phospholipase A2, aiPLA2, Antioxidant Protein 2, AOP2, AOP2-rs3, Brp-12, Ciliary Body Glutathione Peroxidase, CP-3, EC 1.11.1

Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Catalytic activity: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. Subunit: Homotetramer. May interact with HTR2A. \n\nCellular Localization:\nCytoplasm. Lysosome. Note=Also found in lung secretory organelles. The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. Belongs to the ahpC/TSA family. Rehydrin subfamily.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3352-33

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Sheep

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

P Type

Pab

Purity

Serum

References

1. Kim T.-S, et al. J. Biol. Chem. 272:2542-2550(1997). ; 2. Choi H.-J, et al. Nat. Struct. Biol. 5:400-406(1998). ; 3. Rush J, et al. Nat. Biotechnol. 23:94-101(2005). ; 4. Wagner E, et al. Biochem. J. 366:777-785(2002). ; 5. Chen J.-W, et al. J. Biol. Chem. 275:28421-28427(2000). ; 6. Kang S.W, et al. J. Biol. Chem. 273:6303-6311(1998). ; 7. Gevaert K, et al. Nat. Biotechnol. 21:566-569(2003). ; 8. Gregory S.G, et al. ature 441:315-321(2006). ; 9. Nagase T, et al. DNA Res. 2:37-43(1995). ; 10. Frank S, et al. Oncogene 14:915-921(1997). ; 11. Power J, et al. Am J. Path. 161: 885-894 (2202). ; 12. Power J, et al. Experimental Lung Res. 25: 379-391(1999).

Additional Info

This antibody has been shown to be specific for Peroxiredoxin-6 protein. Species Crossreactivity: Rat, human and mouse