PERP (p53 Apoptosis Effector Related to PMP-22, Keratinocyte-associated Protein 1, KCP1, KCP-1, KRTCAP1, P53-induced Protein PIGPC1, PIGPC1, Transmembrane Protein THW, THW)

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID:MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22:gas3 and is a new member of the PMP-22:gas3 family.\n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 10ug/ml\nWestern Blot: 0.5-1ug/ml\nImmunocytochemistry: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nA431 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3353-01H

Size

50ug

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7. 2. Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9. 3. Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288(5468):1053-8. 4. Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62. 5. Lin Y, Ma W, Benchimol S. Pidd, a new death-domain-containing protein, is induced by p53 and promotes apoptosis. Nat Genet. 2000;26:122-7. 6. Luo J, Su F, Chen D, et al. Deacetylation of p53 modulates its effect on cell growth and apoptosis. Nature. 2000;408:377-81. 7. Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein (MTBP) binds to MDM2 and induces a G1 arrest that is suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90. 8. Attardi,L.D., Reczek,E.E., Cosmas,C., et al. PERP, an apoptosis-associated target of p53, is a novel member of the PMP-22/gas3 family. Genes Dev. 2000;14 (6):704-718. 9. Goltsov,A.A., Ren,C., Wang,J., et al. p53 regulates the expression of a novel four transmembrane protein-PERP/PIGPC1 in mouse and human prostate cancer. GeneBank database. 10. Hildebrandt,T., Weidle,U., Preiherr,J. et al. Identification of THW, a putative new tumor suppressor gene. GeneBank database.

Additional Info

Recognizes human PERP.

Alternative Names

PERP, TP53 Apoptosis Effector, 1110017A08Rik, dJ496H19.1, RP3-496H19.1