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PF4 (Platelet Factor 4) (Biotin)

Cat no: P3360A

PF4 (Platelet Factor 4) (Biotin)

Applications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: To detect hPF-4 use at a concentration of 0.15-0.3ug/ml (100ul/well antibody solution). Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hPF-4.\n\nWestern Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.\n\nOptimal dilutions to be determined by the researcher.\n\nReconstitution:\nReconstitute to a concentration of 50ug/ml with sterile PBS containing 0.1% BSA. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nCountry of Origin:\nUSA

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SPECIFICATIONS

Catalog Number

P3360A

Size

25ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder in PBS, pH 7.4

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human PF4.

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