Phage Coat Protein g8p

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities [1, 2]. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood. The possibility to select from human combinatorial libraries and the easy way by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to filamentous coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.\n\nApplications:\nSuitable for use in Immunohistochemistry, Western Blot. Immunocytochemistry, Flow Cytometry, ELISA and Affinity Chromatography. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (ABC): 1:25-1:200\nFlow Cytometry: 1:25-1:200\nWestern Blot: 1:100-1:1000\nOptimum dilutions to be determined by researcher.\n\nHybridoma:\nSP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. rther dilutions can be made in assay buffer.

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