PHAP, NT, Control Peptide (Putative HLA DR-associated Protein, Putative Human HLA Class II Associated Protein, Acidic (Leucine-rich) Nuclear Phosphoprotein 32 Family Member B, ANP32B, Acidic Leucine-rich Nuclear Phosphoprotein 32 Family Member A, ANP32A,

Synthetic peptide corresponding to amino acids at amino terminus of human PHAP (1). Corresponding antibody detects all three identified isoforms of PHAP: PHAP I, PHAP I2a and PHAP III. Species Crossreactivity: Species Crossreactivity: human, mouse and rat.\n\nApoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3,-6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins ( PHAPs ) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras and Myc-mediated cell transformation.\n\nApplications: \nSuitable for use in ELISA, Western Blotting. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: The peptide is used for blocking the activity of PHAP antibody. It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37?C. \n\nOptimal dilutions to be determined by the researcher.\n\nCorresponding antibody: P3368-01\n\nPositive Control: Raji cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3368-01A

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS pH 7.2 (10 mM NaH2PO 4, 10mM Na 2HPO4, 130mM NaCl), 0.1% BSA, 0.02% sodium azide.

Purity

Purified

References

Specific Product Reference:Scarabelli, T.M. et al. (2002) Circ. Res. 90:745-748.\n\nGeneral;\n1. Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway. Science. 2003 ;299 (5604):223-6. 2. Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10 ;299 (5604):214-5