Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (FITC)

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. Thesy can be used to select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.\n\nSpecificity: \nRecognizes a complex branched oligosaccharide structure. Not inhibited by simple sugars.\n\nActivity: \n(same/less than) 5ug/ml will agglutinate Type O human erythrocytes.\n(same/less than) 0.5mg/ml will agglutinate neuraminidase-treated erythrocytes.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.\n\nAlso Available:\nP3370-10: Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (Agarose)\nP3370-11: Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (Biotin)\nP3370-20: Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (FITC)\nP3370-25: Phaseolus vulgaris E (PHA-E, Red Kidney Bean) (HRP)

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SPECIFICATIONS

Catalog Number

P3370

Size

1mg

Applications

IHC

Form

Supplied as a liquid in 0.02M sodium bicarbonate, pH 9.0-9.5, sodium azide.Supplied as a liquid in 0.02M sodium bicarbonate, pH 9.0-9.5, 0.1% sodium azide. Labeled with FITC. FITC: Protein (OD495/OD280) = 1.6

Purity

Highly purified (SDS-PAGE).

References

1. Kaifu, R., et al., Carbohydrate Res. 52: 179-185 (1976).

Alternative Names

Kidney Bean; Phytohemagglutinin