PHD2 (Prolyl Hydroxylase Domain Containing Protein 2, PHD-2, Chromosome 1 Open Reading Frame 12, C1orf12, DKFZp761F179, ECYT3, EGL-9 Homolog of C. elegans 1, EGL Nine (C. elegans) Homolog 1, Egl Nine Homolog 1, EGLN1, HIF Prolyl Hydroxylase 2, HIF-PH2, HI

HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.\nHPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.\n\nApplications: \nSuitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry.. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10-1:100\nWestern Blot: 1:500-1:2500. Detects a band at 46-50kD\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3374-01D

Size

100ul

Applications

FC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bruick, RK, McKnight, SL. A Conserved Family of Prolyl-4-Hydroxylases That Modify HIF. Science. 294: 1337-1340 (2001) 2. Ivan, M, et al. Biochemical Purification and Pharmacological Inhibition of a Mammalian Prolyl Hydroxylase Acting on Hypoxia-Inducible Factor. PNAS. 99(21): 13459-13464 (2002) 3. Maxwell, PH, Ratcliffe, PJ. Oxygen Sensors and Angiogenesis. Cell & Dev. Bio. 13: 29-37 (2002)

Additional Info

Recognizes human PHD2. Recognizes an epitope mapped to a region between residues 1 and 50 of human PHD2 (NP_071334.1) (GeneID 54583). Does not react with mouse.