Phosphatase, Alkaline, Liver, Bone, Kidney (Akp2, ALPI, ALPL)

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This antibody can be used to monitor the expression of the human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by Flow Cytometry.\n\nApplications: \nSuitable for use in Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 5-20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nUndifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4071-18

Size

100ug

Applications

FC, ICC, IF, IP

Hosts

Mouse

Reactivities

Hum, Fel, NHP, Prc, Rabbit

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Andrews, P.W., et al., Hybridoma 3: 33-39 (1984). 2. Swallow, D.M., et al., Ann. Hum. Genet. 50: 229-235 (1986). 3. Draper, J.S., et al., J. Anat. 200: 249-258 (2002). 4. Henderson, J.K., et al., Stem Cells 20: 329-337 (2002). 5. Andrews P.W., et al Int. J.Cancer. 66(6): 806-816 (1996).

Additional Info

Recognizes human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP). Does not crossreact with other isoforms of alkaline phosphatase. Species Crossreactivity: porcine, feline, tiger, rabbit, higher primates (chimpanzee, gorilla, orangutan, gibbon), and New World monkeys (owl monkey, squirrel monkey). No crossreactivity has been observed with bovine, canine, goat, guinea pig, hamster, rat, mouse, sheep and Old World monkeys (baboon, Rhesus monkeys, mangabey, mandrill, Celebese black ape, African green monkey).