Phosphatase, Alkaline, Liver, Bone, Kidney (AP)

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by post-translational modification. Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the human Liver/Bone/ Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.\n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 5-20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nUndifferentiated human embryonic stem cells (H9 line), NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4071-18A

Size

100ug

Applications

FC, IF, IP

Hosts

Mouse

Reactivities

Hum, NHP

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Miki, T., et al., Stem Cells 23: 1549-1559 (2005). 2. Oh, S.K., et al., Stem Cells 23: 211-219 (2005). 3. Swallow, D.M., et al., Ann. Hum. Genet. 50: 229-35 (1986). 4. Henderson, J.K., et al., Stem Cells 20: 329-337 (2002). 5. Draper, J.S., et al., J. Anat. 200(3): 249-258 (2002). 6. Andrews, P.W., et al., Hybridoma 3(1): 33-39 (1984). 7. Andrews, P.W., et al., Int. J. Cancer 66(6): 806-816 (1996).

Additional Info

Recognizes human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP). Crossreactivity: Liver/Bone/Kidney AP isozyme from higher primates (chimpanzee, gorilla, orangutan and gibbon) and Old World Monkeys AP (baboon, Rhesus monkey, mangabey, mandrill, celebese black ape, african green monkey). Does not crossreact with New World Monkeys (owl monkey, squirrel monkey), bovine, canine, feline, goat, guinea pig, hamster, mouse, porcine, rabbit, rat, sheep and tiger. Does not crossreact with other isozymes of AP.