Phosphatidylserine (PS)

Phosphatidylserine (PS) is a phospholipid found in the membrane of all plants and animals. PS is most concentrated in the brain, as PS makes up about 10% of the lipid content of neurons. Neural PS is thought to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. One of the key features of apoptosis is the expresion of PS on the outer cell membrane. PS is usually found on the inner side of the plasma membrane, but during early stage apoptosis, the PS is translocated to the outer membrane. FITC-labeled annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays.\n\nApplications: \nSuitable for use in Immunohistochemistry and Flow Cytometry. Other applications have not been tested.\n\nRecommended Dilutions:\nImunohistochemistry: Reported to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone.\nFlow Cytometry: 0.2-2ug/ml detects cell surface phosphatidylserine on Jurkat cells treated with Fas Ligand vesicles. \nDose response of Fas Ligand using surface phosphatidylserine \n(PS) as readout: Jurkat cells were treated with increasing doses of Fas Ligand, Membrane Bound. After 18 hours, cells were surface stained with P4071-80, (2ug/ml) followed by an IgG F(ab

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SPECIFICATIONS

Catalog Number

P4071-80

Size

200ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 0.07M Tris-glycine, pH 7.4, 0.105M sodium chloride, 0.035% sodium azide, 30% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

US Biological application reference: Yan, X. et al., (2012) Cancer, Immunology and Immunotherapy, DOI: 10.1007/s00262-012-1250-4. 1. Mandinov, L., et al., Proc. Natl. Acad. Sci. 100: 6700-6705 (2003). 2. Koopman, G., et al., Blood 84: 1415-1420 (1994). 3. Vermes, I., et al., J. Immunol. Methods 184: 39-51 (1995).

Additional Info

Recognizes phosphatidylserine (PS) in cell membranes. Species Crossreactivity: vertebrates