Phosphodiesterase 4A, phosphorylated (Phosphodiesterase 4A cAMP-specific, Phosphodiesterase-4A, PDE4A, cAMP Specific 3

This antibody is ideal for studying phosphorylation dependent signal transduction research of PDE4 activation.\n\nCellular Localization: Cell Membrane\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4072-03D

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Rabbit

Form

0.02% Thimerosal / Merthiolate

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

This product has been used in:Zhu B et al. Activation and induction of cyclic AMP phosphodiesterase (PDE4) in rat pulmonary microvascular endothelial cells. Biochem Pharmacol 68:479-91 (2004). <PUBMED:15242814> Zhang HT et al. Inhibition of cyclic AMP phosphodiesterase (PDE4) reverses memory deficits associated with NMDA receptor antagonism. Neuropsychopharmacology 23:198-204 (2000). <PUBMED:10882846>Yarwood SJ et al. The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform. J Biol Chem 274:14909-17 (1999). <PUBMED:10329691>Beavo JA Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol Rev 75:725-48 (1995). <PUBMED:7480160>

Additional Info

This antibody detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phosphorylation site. It will not crossreact with PDE4A phosphorylated at the ERK phosphorylation site. It does not crossreact with PDE4B, PDE4D or any other member of the PDE family. Species Crossreactivity: Human (weakly), Mouse, Rat and Rabbit.