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Phosphodiesterase 4B (PDE4B)

Cat no: P4072-03

Phosphodiesterase 4B (PDE4B)

Of the 11 families of phosphodiesterase (PDEs) identified, PDE4, PDE7, and PDE8 are specific for cAMP. However, isoforms of the PDE4 family often account for most of the cAMP-hydrolyzing activity of a cell. Four genes (PDE4A, B, C and D) make up the PDE4 family. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In the CNS, PDE4B subtype variants are expressed in varying ratios. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants.\n\nApplications:\nSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.\n\nRecommended Dilution;\nImmunoprecipitation: 7ug/ml\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4072-03

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Reacts with the human and rat PDE4B protein. On Western blots, it identifies a single band at ~83kD. Bands of unknown origin may be observed at ~160 and ~260kD. Reactivities have been confirmed with NG96 human neuroblastoma lysate and rat brain homogenate.

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info
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