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Phosphodiesterase 4D3, Recombinant, Human, GST-Tag (PDE4D3)

Cat no: P4072-03W

Phosphodiesterase 4D3, Recombinant, Human, GST-Tag (PDE4D3)

Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5; 1, 2). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.\n\nDescription:\nRecombinant human PDE4D3 (GenBank Accession No. NM_006203, full length, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system. \n\nSpecific Activity:\n~5000pmol/min/ug\n\nAssay Conditions: \n10mM Tris-HCl, pH 7.4, 10mM MgCl2, 1mM MnCl2, 200uM cAMP, 0.1mg/ml BSA, 0.2ng/ul P4072-03W at 37C for 20 minutes.\n\nApplication: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. \n\nRecommended Dilutions: \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4072-03W

Size

5ug

Form

Supplied as a liquid in 50mM Tris, pH 8.0, 138mM sodium chloride, 2.7mM potassium chloride, 3mM DTT, 0.05% Tween-20, 10% glycerol

Purity

(same/more than)70%

References

1. Fidani,L., et al., Eur. J. Neurol. 14 (7), 745- 749 (2007). 2. Baillie,G.S., et al., Biochem. J. 404 (1), 71-80 (2007).

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