Phosphodiesterase Type 4D7 (PDE4D7)

Applications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:10,000\nImmunoprecipitation: 1-4ug/mg\nFlow Cytometry: 1.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4072-03R

Size

100ug

Applications

ELISA, FC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hoffmann, R. et al., The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579. EMBO J 18:893-903 (1999). 2. Erdogan, S .& Houslay, M.D., Challenge of human Jurkat T-cells with the adenylate cyclase activator forskolin elicits major changes in cAMP phosphodiesterase (PDE) expression by up-regulating PDE3 and inducing PDE4D1 and PDE4D2 splice variants as well as down-regulating a novel PDE4A splice variant. Biochem J 321 ( Pt 1):165-75 (1997). 3. Bolger, G.B. et al. Characterization of five different proteins produced by alternatively spliced mRNAs from the human cAMP-specific phosphodiesterase PDE4D gene. Biochem J. 328 ( Pt 2):539-48 (1997). 4. Alvarez, R. et al., Activation and selective inhibition of a cyclic AMP-specific phosphodiesterase, PDE-4D3. Mol Pharmacol 48:616-22 (1995).

Additional Info

Recognizes rat Phosphodiesterase Type 4D7. Does not crossreact with PDE4D1, D2, D3, D4 or D5 variants. It also does not cross react with PDE4A, PDE4B or PDE4C. Species Crossreactivity: human and mouse.