PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.
By expressing the gene encoding human PGM-B and/or PGM-M subunit, the isozymes are separately produced in enzymatically active form. Active recombinant human PGAM-B is produced as a soluble protein in E. coli by expressing the gene encoding PGAM-B.
Application:
Suitable for use as activity Assay of PGM in the presence (PGM-B activity) or absence (total PGM) of K-tetrathionate, which is a potent inhibitor of PGM-M, to diagnose the acute stage of apoplexy or myocardial infection. Other applications not tested.
Specific Activity:
(same/more than)500u/mg protein. One unit will produce micromole of NAD+ per minute at 30 degrees C, determined according to the method described by Bergmeyer, et al. in Methods of Enzymatic analysis (3rd Ed). 1983, pp 282-283.
Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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