Phosphoinositide 3-kinase gamma, p110 gamma, catalytically inactive mutant, Recombinant, Human (PI3K)

The PI3K D946A protein is a catalytically inactive mutant of PI3K with a D946A mutation in the ATP binding site. This recombinant catalytically inactive protein can be used as a negative control in any kind of PI3K kinase activity studies. Recombinant full length PI3K DA mutant carries a N-terminal GST-Tag and was purified by affinity chromatography. The GST-Tag facilitates the protein

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SPECIFICATIONS

Catalog Number

P4073-35F

Size

10ug

Form

Liquid. Supplied in 25mM Tris, pH 8.0, 50mM NaCl, 0.5mM MgCl2 and 50% glycerol.

Purity

(same/more than) 90% by SDS-PAGE

References

Fuchikami et al. (2002) A Versatile High-Throughput Screen for Inhibitors of Lipid Kinase Activity: Development of an Immobilized Phospholipid Plate Assay for Phosphoinositide 3-kinase . J. Biomol. Scr. 7:441.\nLeopoldt D. et al. (1998) Gbetagamma stimulates phosphoinositide 3-kinase-gamma by direct interaction with two domains of the catalytic p110 subunit. J. Biol. Chem. 273:7024.\nLopez-Ilasaca M. et al. (1997) Linkage of G protein-coupled receptors\nto the MAPK signaling pathway through PI 3-kinase gamma. Science 275:394.\nStoyanov B. et al. (1995) Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase. Science 269:690.\nStoyanova S. et al. (1997) Lipid kinase and protein kinase activities of G-protein-coupled phosphoinositide 3-kinase gamma: structureactivity analysis and interactions with wortmannin. Biochem. J. 324:489.\nFoster et al. (2003) The phosphoinositide (PI) 3-kinase family. J. Cell Science. 116:3037.\nKerchner et al. (2004) Differential Sensitivity of Phosphatidylinositol 3-Kinase p110 to Isoforms of G Protein Dimers. J. Biol. Chem. 279:44554.\nDel Prete et al. (2004) Defective dendritic cell migration and activation of adaptive immunity in PI3Kgamma-deficient mice. EMBO J. 23:3505.\nWeiss-Haljiti et al. (2004) Involvement of phosphoinositide 3-kinase gamma, Rac, and PAK signaling in chemokine-induced macrophage migration. J. Biol. Chem. 279:43273.