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Phosphoinositide 3 Kinase, Recombinant, Human, GST-Tag, p110 alpha, p85 alpha (PI3K)

Cat no: P4073-26C

Phosphoinositide 3 Kinase, Recombinant, Human, GST-Tag, p110 alpha, p85 alpha (PI3K)

Phosphoinositide 3 Kinase, Recombinant, Bovine, p85 alpha (PI3K) functions as the regulatory subunit of the class IA PI3-kinase isoforms a, b, and d. It contains two SH2 domains that bind to tyrosine-phosphorylated growth factor receptors or substrate adaptor proteins. It also contains a BH (breakpoint cluster region homology) domain that shows GAP activity towards the small GTPases Rab4, Rab5, Cdc42, Rac1 and to a lesser extend towards Rab6 and Rab11. It was shown that PI3Ka catalytic subunit mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3'-phosphorylated phosphoinositides. PI3Ka protein kinase activity has been implicated in IRS-1 serine phosphorylation in insulin-treated adipocytes and in STAT3 and IRS-1 phosphorylation upon activation of the type 1 IFN receptor by IFN-a. \n\nComplex of N-terminal GST-tagged recombinant full-length human p110 alpha (GenBank Accession No. U79143), and N-terminal His tagged, recombinant full length, human p85alpha (XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.\n\np110 alpha MW 129kD, p85 alpha MW 88kD. \n Form: Supplied as a liquid in 25mM Tris, pH 8.0, 69mM sodium chloride, 1.35mM KCl, 0.025% Tween-20, 3mM DTT, 50% glycerol\n\nApplications:\nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nAssay Buffer:\n40mM Tris-HCl, pH 7.5, 20mM MgCl2, 100uM ATP, 200uM PI/PS, 20-100ng P4073-26C for 30 min at 30C.\n\nSpecific Activity:\n~600pmol/min/ug\n\nStorage and Stability:\nFor long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months. \n

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SPECIFICATIONS

Catalog Number

P4073-26C

Size

20ug

Reactivities

Hum

Form

Supplied as a liquid in 25mM Tris, pH 8.0, 69mM sodium chloride, 1.35mM KCl, 0.025% Tween-20, 3mM DTT, 50% glycerol

Purity

(same/more than)80% by SDS-PAGE

References

1. Vogt, PK et al., Virology. 344(1):131-8(2006). 2. Bader, AG etal., Nat Rev Cancer. Dec;5(12):921-9 (2005).

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