Phospholipase A2, Secretory, Group IB, Recombinant, Human (PLA2, sPLA2-IB)

Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentrationdependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.\n\nDescription: \nThe Recombinant Human Secreted Phospholipase A2-IB is manufactured with N-terminal fusionf HisTag. The Recombinant Human Secreted Phospholipase A2-IB His-Tagged Fusion Protein is 16kD containing 126 amino acid residues of the human secreted phospholipase A2-IB and 16 additional amino acid residues-HisTag.\n\nAmino Acid Sequence: \nMRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS\n\nSpecificity: \nThe amino acid sequence of the recombinant human Secreted Phospholipase A2-IB is 100% homologous to the amino acid sequence of the human Secreted Phospholipase A2-IB.\n\nApplications: \nWestern Blot\n\nReconstitution: \nAdd 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.\n\nStorage: \nStore lyophilized protein at -20 degrees C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degrees C for a limited period of time; it does not show any change after two weeks at 4 degrees C.

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SPECIFICATIONS

Catalog Number

P4074-06D3

Size

2ug

Form

Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0.

Purity

> 95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.

References

1. Expression and induction of secretory phospholipase A2 group IB in brain.\n\nCell Mol Neurobiol 2005 Nov;25(7):1107-22\n\n2. Group IB secretory phospholipase A2 stimulates leukotriene B4 production by a unique mechanism in human neutrophils.\n\nBiochem Biophys Res Commun 2005 Aug 26;334(2):500-8\n\n3. Group IB secretory phospholipase A2 stimulates CXC chemokine ligand 8 production via ERK and NF-kappa B in human neutrophils.\n\nJ Immunol 2004 Nov 15;173(10):6433-9\n\n4. The role of propeptide in the refolding of human group IB phospholipase A2.\n\nActa Biochim Biophys Sin (Shanghai) 2004 Sep;36(9):583-8\n\n5. Group IB secretory phospholipase A2 promotes matrix metalloproteinase-2-mediated cell migration via the phosphatidylinositol 3-kinase and Akt pathway.\n\nJ Biol Chem 2004 Aug 27;279(35):36579-85\n\n6. Comparative analysis of gene expression mechanisms between group IA and IB phospholipase A2 genes from sea snake Laticauda semifasciata.\n\nGene 2004 May 12;332:179-90