Phospholipase C, gamma 1 (PLCg1)

Phospholipase C gamma 1 is a tryosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-triphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLC gamma activity is unknown.\n\nApplications: \nSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.5ug/ml. Detects PLCg1 in acid precipitated protein from bovine brain, RIPA lysates from A431 cells, and lysates from 3Y3/A31 fibroblasts and L6 myoblasts.\nImmunoprecipitation: 4ug (bovine brain cytosol).\nOptimal dilutions to be determined by the researcher.\n\nPositive Antigen Control (Included): A0001-50 (Non-stimulated A431 cell lysate). Add 2.5ul of 20mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Use 20ug per lane for minigels.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding ddH2O and 40-50% glycerol. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4074-42C

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Rabbit

Form

Supplied as lyophilized powder from 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. Reconstitute with 100ul dH2O, 40-50% glycerol.

P Type

Mab

Purity

Purified by Protein G chromatography.

Isotype

IgG1

References

1. Suh, P.G., et al., J. Biol. Chem. 263: 14497

Additional Info

Recognizes bovine PLCg-1 at ~135kD. May also react with some non-PLC g-1 SH-3 containing proteins(4). Species crossreactivity: rabbit, rat, mouse and human.