Phospholipase C, gamma 2, phosphorylated (Y1197) (PLCg2)

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCgII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgII (2). Btk-deficient B cells exhibited a marked reduction in phosphorylation of the Src homology 2 (SH2)-SH3 linker region sites, Tyr(753) and Tyr(759). Phosphorylation of both sites was restored by expression of Tec, but not Syk, family kinases. In contrast, phosphorylation of the PLCgamma2 carboxyl-terminal sites, Tyr(1197) and Tyr(1217), was unaffected by the absence of functional Btk (3). \n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.\nRecommended Dilutions:\nWestern Blot: 1:2000-1:20,000 \nImmunocytochemistry: 1:100-1:250 \nImmunoprecipitation: 1:40 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

P4074-45E

Size

100ul

Applications

ELISA, ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Argeson AC, et al. Genomics 25(1):29-35, 1995. 2. Ozdener F, et al. Mol Pharmacol 62(3):672-9, 2002. 3. Humprhries LA, et al. J Biol Chem 279(36):37651-61, 2004

Additional Info

Recognizes human PLCg2 when phosphorylated on tyrosine 1197.