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Phospholipid Assay Kit, BioAssay(TM)

Cat no: P4074-60

Phospholipid Assay Kit, BioAssay(TM)

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. This method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.\n\nKey Features:\nSensitive. Use 20ul samples. Linear detection range: Colorimetric Assay: 3-200uM Fluorimetric Assay: 0.6-20uM phospholipid.\n\nApplications:\nAssays: phospholipid in biological samples such as serum and non-EDTA plasma.\nDrug Discovery/Pharmacology: effects of drugs on choline-containing phospholipid metabolism.\n\nKit Components:\n1. Assay Buffer, 1x10ml\n2. Enzyme Mix, 1x 120ul\n3. PLD Enzyme, 1x120ul\n4. Dye Reagent, 1x120ul\n5. Standard phosphatidylcholine (2mM), 1x400ul\n\nStorage and Stability:\nStore at -20 degrees C. Stable for 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4074-60

Size

1Kit

References

1. Nie, Y. et al. (1993). A micro enzymic method for determination of choline-containing phospholipids in serum and high density lipoproteins. Lipids. 28: 949-951.\n2. Grohganz, H. et al. (2003). Quantification of various phosphatidyl-cholines in liposomes by enzymatic assay. A APS Pharm. Sci. Tech. 4(4): E63.\n3. Hojjati, MR, Jiang XC (2006). Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res. 47: 673-676.

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