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Phosphotyrosine Control, EGF-Stimulated A431 Cell

Cat no: P4078-10

Phosphotyrosine Control, EGF-Stimulated A431 Cell

Applications: \nPositive control for anti-phosphotyrosine antibodies. Suitable for use in Western Blot, Immunoprecipitation.\n\nRecommended Dilution:\nWestern Blot: Detects the phosphorylated EGF receptor (170kD) and other tyrosine phosphorylated proteins in 20ug of the preparation. Add an equal volume of Laemmli reducing sample buffer to 5-25ul of lysate a;nd boil for 5 min to reduce the preparation.\n\nImmunoprecipitation Positive Control: This preparation may also be used for immunoprecipitation with anti-phosphotyrosine agarose. \n\nStorage and Stability:\nStore at -20C Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

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SPECIFICATIONS

Catalog Number

P4078-10

Size

1mg

Applications

IP, WB

Form

Supplied as a frozen solution in RIPA sample buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM Na3VO4, 1mM NaF).

Purity

Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor.

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